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Several technical reasons may explain our distinctive findings, including differences in the microbiome sampling site, the limited n per group, singly housing mice, or the use of highly similar lean, Western, and high-fat diets which systematically exchanged fat content with carbohydrate content
The sample was treated with a solution of glacial acetic acid (20% v/v), sodium dodecyl sulfate (8.1% v/v), and thiobarbituric acid (0.8% w/v) before being incubated for 1 h at 95 C in a water bath
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Clinically, this results in increased blood pressure and heart rate due to unopposed sympathetic stimulation
Second, the BDNF and NGF mRNA upregulation triggered by a single dose continues for 12 to 24 hours and drives sustained behavioural change
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