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doi:10.1186/s12951-018-0392-8
Furthermore, inclusion of a His-tag during expression (for subsequent Ni-nitrilotriacetic acid purification) might alter protein folding and thus binding results (Ledent et al., Table 2)
In Plutella xylostella , the amino acids Phe-9, Pro-10, Ile-11, Leu-14, Gly-49, Pro-52, Ala-100, and Tyr-107 are the putative H-site residues in a sigma class GST, PxGST [77]
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