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coli mutants Bacterial colonies from individual bacterial mutants were streaked on LB agar plates, grown at 37 C, and genotyped by PCR using the kanamycin-cassette-specific primers (forward primer 5-CGGTGCCCTGAATGAACTGC-3, reverse primer 5-CAGTCATAGCCGAATAGCCT-3) and genomic primers for individual bacterial mutants, which were designed at starting 100-300 bases upstream of start-codon (forward primer) or downstream of stop codon (reverse primer)

Takemura K, Kawachi H, Eishi Y, Kitagaki K, Negi M, Kobayashi M, et al

Eligibility criteria We included RCTs with a duration of intervention of at least 12 weeks that compared tirzepatide at a maintenance dose of 5, 10 or 15 mg once-weekly, administered subcutaneously, with placebo or any other glucose-lowering medication

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