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Beads were washed with WB1 (50 mM HEPES-KOH, 150 mM NaCl, 1 mM EDTA, 1% sodium deoxycholate, 1% Triton X-100, 0.1% SDS, 1 mM PMSF, pH 8.0), WB2 (50 mM HEPES-KOH, 1 M NaCl, 1 mM EDTA, 0.1% Na deoxycholate, 1% Triton X-100, 0.1% SDS and 1 mM PMSF, pH 8.0), WB3 (50 mM Tris, 0.25 mM LiCl, 1 mM EDTA, 0.5% NP-40 and 0.5% Na deoxycholate, pH 8.0), and TE