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RNA yield and integrity were initially assessed from absorbance readings at 260 and 280 nm using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, Loughborough, UK), and confirmed using an Agilent Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent Technologies, Wokingham, UK) according to the manufacturers guidelines

However, especially under high stress from environmental influences, sports, or illness, the glutathione level can decrease

Handling protocols require following institutional guidelines for research chemicals

Now, among anti-aging treatments, glutathione injectable treatments stand out

When the data did not meet these assumptions, non-parametric tests were applied, namely the Kruskal-Wallis test followed by Dunns post hoc test