At the end of fermentation, the biomass was centrifuged, the supernatant was discarded, and the pellet was resuspended in 100 mL of a solution composed of 20% maltodextrin as a cryoprotectant agent
Tirzepatide (Mounjaro, Zepbound) is a related but distinct medication that acts as a dual glucose-dependent insulinotropic polypeptide (GIP) and GLP-1 receptor co-agonist
Thermostability and binding measurement suggest that introducing pAcF not only provides a chemical handle for conjugation but also facilitates in vitro affinity maturation and thermostability improvement, leading to the construction of BsNbs with enhanced binding efficacy and thermostability
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Segura-Pena, D., Sekulic, N., Ort, S., Konrad, M