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GCL activity was calculated as the difference in GSH concentration between the reaction and baseline samples per minute normalized to the protein content in the sample

Complex IV is composed of a total of 13 protein subunits

HaCaT keratinocytes have also been proven to be good candidates for assessing skin irritancy and toxicity in conventional monolayer assays measuring using MTT reduction as the viability endpoint 21

If you smoke and consume alcohol then avoid them at least 2 weeks before treatment

Enzymatic assay for glutathione