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found that GLP-1 RAs are associated with an increased risk of gallbladder and biliary diseases (including cholelithiasis and cholecystitis), particularly at higher doses, longer treatment durations, and when used for weight loss [129]

Comet assay For each animal, a portion of the left lobe of the liver was placed into a tube containing cold mincing solution [Mg2+ and Ca2+ free Hanks Balanced Salt Solution (Invitrogen, Carlsbad, CA) containing 10% v/v DMSO, and 20 mM EDTA pH 7.4-7.7] and rapidly minced