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Finally, existing challenges to brain drug delivery using nanoparticles are presented, such as the accumulation of nanoparticles in the brain, which can produce neurotoxicity, and the lack of preclinical CNS disease models that can mimic the microenvironment of the human brain relatively perfectly

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HPA axis studies measure serum cortisol, ACTH, and CRH levels via radioimmunoassay or ELISA following DSIP administration in stress-challenged animal models

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