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In vitro studies confirm that linagliptin suppresses the proliferation of several tumor cell lines, including colorectal cancer HCT116, breast cancer MCF-7, and liver cancer HepG2, via mechanisms that involve cell cycle arrest and apoptosis induction [96]

These technologies potentially allow the setup of an in vitro test system, which resembles the in vivo situation as closely as possible 27

This approach introduces dose-response variability that undermines experimental precision, making data interpretation impossible

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