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Research use only SLU-PP-332 research peptide SLU-PP-332 is a synthetic small-molecule pan agonist of the estrogen-related receptors ERRalpha, ERRbeta, and ERRgamma, with the highest reported potency at ERRalpha

GPX4 specific activity was carried out in a coupled assay, by monitoring the consumption of NADPH in the presence of glutathione reductase upon addition of phosphatidyl choline hydroperoxide ( Viability Assay Cells were seeded onto 96-well plates (5,000 cells per well) and allowed to attach overnight

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Conclusion: Altogether, our results show that the cellular antioxidant defense systems contribute to leukemia resistance to MTX, and targeting these pathways, especially the thioredoxin antioxidant system, may be a promising strategy for resensitizing ALL to MTX