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The method optimization takes into account the functional data of GP complex: 1) the peroxilipid is reduced by GSH (reduced glutathione) to hydroxilipids (GP activity)
BMC Endocrine Disorders, 23, 256
Science 337(6100):13571360 Schiavone S, Sorce S, Dubois-Dauphin M, Jaquet V, Colaianna M, Zotti M, Cuomo V, Trabace L, Krause KH (2009) Involvement of NOX2 in the development of behavioral and pathologic alterations in isolated rats
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Thus, structures of glutathionylated glutaredoxins 31,43,44,45 most likely resemble enzyme species F, whereas similar structures of complexes between reduced glutaredoxin and GSH with longer sulfursulfur distances 24,46 do not represent the interaction with GSH (but probably also resemble species F)
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