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After blocking with 5% bovine serum albumin (BSA) for 1 h, the cells were incubated overnight at 4 C with -H2A.X antibody (Cell Signaling Technology, #2577, 1:1000), followed by washing and incubation with the appropriate fluorescent secondary antibody for 1 h at room temperature

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Research on Epithalon has centered heavily on telomerase activation several published studies report that the peptide can increase telomerase activity in human somatic cell lines, a mechanism directly tied to telomere length and cellular replicative capacity

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