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The coupling agent used in the reaction can be selected from group consisting of [Ethylcyano (hydroxyimino)acetate-02)-tri-(l-pyrrolidinyl)-Phosphonium hexa fluorophosphate (PyOxim), ethyl-2 -cyano-2-(hydroxy amino) acetate (Oxyma pure), 0-(benzotriazol-l-yl)-N,N,N,N'- tetramethyluronium tetrafluoroborate (TBTU), diisopropyl carbodiimide (DIC), 1,3- dicyclohexylcabodiimide (DCC) , 0-(7 -azabenzotriazol- 1 -yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), 1 -(dimethyl aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC HC1), 0-(benzotriazol-l-yl)-l,l,3,3-tetra methyluronium hexafluorophosphate (HBTU), 1- Hydroxybenzotriazole (HOBt), Isopropyl chloro formate (IPCF), Benzotriazol-l-yl-oxy- tris(dimethyl-amino)-phosphonium hexa fluorophosphate (BOP), benzotriazole-1- yloxytri(pyrrolidino)phosphonium hexa fluoro phosphate (PyBOP), N,N-bis-(2-oxo-3- oxazolidinyl)phosphonic dichloride (BOP-C1), bromotri(pyrrolidino)phosphonium hexa fluoro phosphate (PyBrOP), 0-(6-Chloro-l-hydrocibenzotriazol-l-yl)-l,l,3,3-tetramethyl uranium tetra fluoroborate (TCTU), chlorotri (pyrrolidino)phosphonium hexafluorophosphate (PyClOP), Ethyl l,2-dihydro-2-ethoxyquinoline-carboxylate(EEDQ), isobutyl chloro formate (IBCF), 2- succinimido-l,l,3,3-tetramethyluronium tetrafluoroborate(TSTU), l-Cyano-2-ethoxy-2-oxo ethylidene aminooxy) dimethyl amino morpholino-carbeniumhexafluorophosphate (COMU), 2- (5-norbornen-2,3-dicarboximido)-l , 1 ,3,3-tetramethyluronium tetrafluoroborate (TNTU), propane phosphonic acid anhydride (PPAA), 3-(diethoxy phosphoryloxy)-l,2,3-benzotriazin- 4(3H)-one (DEPBT) or its mixture

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Peer review Peer review information Communications Biology thanks Chao Mao and the other, anonymous, reviewer(s) for their contribution to the peer review of this work