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Yet, langerin expression in MDLCs is a dynamic continuum that can be further increased depending on the in-vitro culture conditions, for instance in the absence of serum or following short exposure to certain cytokines 42,43

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Two ideas run through this entire guide, and it is worth naming them before the numbers begin

At a 5 mg / 1 mL reconstitution (5 mg/mL common from research vendors who use less BAC water), divide each volume by 2.5: 250 g becomes 0.05 mL, 500 g becomes 0.10 mL