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2.12 Immunofluorescence The aorta (5 microns thick) was subjected to antigen retrieval by heating it in 10 mM citrate buffer (pH 6.0) at 95 C for 10 min after decalcification and hydration

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Any discussion of aging, tissue models, inflammation, or structure-related pathways refers only to research context

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For the expression of full-length AtSNX1 and AtSNX2a, the full-length coding sequences of Arabidopsis SNX1 (AT5G06140) and Arabidopsis SNX2a (AT5G58440) were subcloned into the pET28a-His6-SUMO vector

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