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The bulkier Phe173 4.60 and the inward orientation of Leu249 5.40 effectively occlude Cavity II of the motilin receptor and prevent ligand penetration
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In addition, the isoprenyl group can react with cysteine thiol and bind to proteins, thus forming irreversible S -prenylation reactions such as farnesylation
Nature 551, 247250
To prove that YBX1 can directly bind to PRKAA1 5UTR, a biotinylated 5UTR-specific RNA probe was used to pull down whole-cell lysates and YBX1 was clearly detected in the immunoprecipitant by immunoblots (Extended Data Fig
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