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Blots were blocked with phosphate-buffered saline with 0.1% Tween 20 and then bound with antibodies for Biotin, after which an enhanced chemiluminescence (ECL) reaction was performed for blot development
The blood glucose was measured before (0 minutes) and after 15, 30, 60, and 120 minutes of glucose/insulin administration, using Truetrack glucometer (Nipro Diagnostics), as also described in our earlier studies (15, 41, 74)
Example: The 200 lb Patient If you weigh 200 lbs, heres what a 15% loss looks like: 15% of 200 lbs = 30 lbs
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At the same dose, a 74 kg participant had about 1.4 times the exposure of a 110 kg reference, while a 143 kg participant had about 0.8 times. Weight alone does not set the dose, but it helps explain why the trial mean transfers poorly to bodies outside the studied range
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