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Data for each double hit was obtained from the database and promiscuity was calculated as the percent of assays reported as active ( Table 1 )

Once internal standard concentrations were obtained, the peak areas of the unlabelled metabolites in the samples of culture medium were compared with the peak area of the quantified internal standard to determine the metabolite concentration in the sample

The synthesis and characterization of compounds 112 and PRP are given in Supplementary Methods, Supplementary Figs