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In brief, HCT116 cells were infected with the GeCKO v1 human CRISPR knockout pooled library using lentiviral transduction at a multiplicity of infection of 0.3, and populations stably expressing gRNAs were selected for using puromycin and expanded for 8 days to allow for essential gene drop-out

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The potential effects of red blood cell disorders on male reproduction should be considered, and reproductive urologists should be involved in the comprehensive care of these patients

This ongoing evaluation helps ensure that patients receive optimal benefits while maintaining safety standards throughout their treatment journey