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The isolated metatarsals were cultured in 24-well culture plates in 150 L of DMEM supplemented with 10% heat-inactivated fetal bovine serum and 1% of penicillin/streptomycin and 1% L -glutamine for 72 h
The samples were collected, drop-cast onto Formvar-coated copper grids, dried, and then visualized at predetermined time points (0, 1, 3, 7, 10, and 14 d)
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