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However, the role of inflammasomes in this injury remains unclear, as conflicting findings suggest inflammasomes may exacerbate liver injury

Furthermore, a mechanistic link between ER stress and autophagy suggests that ER stress and unfolded protein response pathways can induce the expression of autophagy-related protein by activating ER stress sensors 16

Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)

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