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Tan, uneven patches, and surface discolouration begin to resolve

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This creates a planning problem most researchers don't anticipate until they're staring at leftover solution approaching its expiration

75 L of the reconstituted samples were injected using a Shimadzu SIL 30AC autosampler into the running solvent (10 mM ammonium acetate in dichloromethane: methanol (50:50, vol/vol)) pumped at an isocratic flow rate of 8 L/min by a Shimadzu Nexera LC30 system

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