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As digital microfluidic technology compacts all steps from sample processing to analysis and waste handling in a disposable cartridge, this fully automated system is an attractive POCT for smaller hospital use when laboratory facilities and trained staff are not available or limited

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Standard curves were prepared in pooled baseline urine from multiple participants collected before intervention, with concentrations ranging from 0 to 16 g/mL for each compound

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Understanding these potential mechanisms helps clinicians and patients identify strategies to minimise discomfort whilst maintaining adequate B12 intake