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Non-specific adhesion sites were blocked for 45 minutes in 0.25% Tween-20/PBS with 1% bovine serum albumin, followed by the addition of primary antibodies against -H2AX (1:10.000, 05-636, Millipore) or RAD51 (1:50, PA5-27195, Thermo Fisher Scientific), or isotype immunoglobulin G in the case of negative controls