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Freeze-thaw cycling is especially destructive to peptides each cycle forms ice crystals that physically shear the molecule, so even one accidental freeze can tank a vial

If they are not, stay put or drop back

This longevity of research interest is itself informative: compounds that fail to replicate across independent laboratories typically disappear from the literature within a decade

As my condition has been so up and down over the years it just gets really complicated, and I can barely explain it to myself let alone someone else

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