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After three washes with MACS flow buffer, the bead-bound-exosomes were resuspended with flow buffer with anti-rabbit IgG with Alexa Fluor 647 (ab150083, Abcam) for 1 h at 4 C

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In contrast, the high-dose proline derivative (200 mg/kg) improved hepatic architecture, similar to silymarin

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When the xenograft volumes were ~50 mm 3 , the mice in all six groups were treated with 100 L of saline buffer or TRAIL (5 g/kg/day) by intraperitoneal injection three times a week for 45 days, and tumor growth was monitored as described above

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