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Ultra-filtration (to wash out un-reacted free trisulfides) Trisulfide-pretreated HGF solutions were loaded into Amicon ultra-filtration devices (with a 10-kDa MWCO polyethersulfone (PES)-membrane) and spun at the recommended centrifugal force (typically 6000-7000 rpm) for 1030 min at 4 or until the desired volume was reached

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Abnormal swelling or jaundice: These could be early signs of liver or gallbladder issues

[5] Preclinical Safety Evaluation of Body Protective Compound-157, a Potential Drug for Treating Various Wounds NIH

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