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The bound Fab was eluted with 25 mM sodium citrate, pH 3.2 and pooled for further purification by size-exclusion chromatography on a Superdex75 column (GE Healthcare) in phosphate buffered saline (PBS)

Symptoms may include: Abdominal pain Jaundice Spike in temperature Gray-colored stool Dont hesitate to contact your doctor if you notice any of these issues

Your healthcare team can provide guidance on managing your diabetes or weight management regimen whilst awaiting replacement medication, including advice on missed doses

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A few hours either way makes little difference with a weekly medication