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In fact, researchers have a name for the fat-to-lean-mass loss ratio that shows up across virtually every serious weight loss method: the quarter fat-free mass rule. It refers to the consistent finding that roughly 25% of weight lost during a significant calorie deficit comes from lean tissue, regardless of how that deficit was created

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To evaluate whether this increase in insulin secretion could be attributed to an increase in cell mass and/or an enhanced secretory capacity of the islets, immunohistochemical analysis of the pancreas and glucose-stimulated insulin secretion (GSIS) assay were performed

For researchers, the choice depends on the question being studied