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For the global metabolite profiling in serum, the metabolites in 10 L of serum were extracted by the 390 L of pre-chilled 82% MeOH (80 C) followed by 15 min incubation at 80 C

NF-KB is sensitive to ROS, and it modulates the transcription of genes encoding endothelin-1, tissue factor, and thrombomodulin (198)

Benavente CA et al

The CPZ signature was strongly enriched in human DAO clusters, supporting the conclusion that CPZ demyelination closely models the transcriptional state of lesion-associated DAOs (Fig