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After 45 min incubation, the resultant biofilms were washed in the appropriate pre-warmed medium (x3), before 1 ml of further medium was added nystatin (1 g/ml) OligoG (4%), with a further 24 h incubation on a rocker at 20 rpm at 37C (n=3)

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In contrast, variant allele 718C of the GPX4 gene was found to be significantly associated with an increased risk of CS in hypertensives (OR=1.53, 95%CI 1.231.90, P =0.0001, P adj =0.0003)