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The mice in the HFHC group were fed a high-trans-fat diet [58 kcal% fat w/sucrose Surwit Diet (D12331, lot number: 17082101A10, Research Diets, USA)] combined with water containing high carbohydrates (55% fructose + 45% sucrose, concentration 42 g/L) to establish an animal model of NASH

These capsules usually have powerful antioxidants like Glutathione, Vitamin C, Alpha-lipoic acid, and other natural ingredients that will help in reducing melanin production, the pigment that is responsible for dark skin

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The project was approved regarding the in vitro models by the NOVA Medical School Ethical Committee (Ref: 158/2021/CEFCM)

Cell 82 , 35533565.e3555 (2022)