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Biotin proximity tagging favours unfolded proteins and enables the study of intrinsically disordered regions
High-performance LC-MS/MS was used to perform high-sensitivity, wide-coverage, and high-throughput HM700-targeted quantification of 700 metabolites in these samples ( In detail, appropriate amounts of experimental or quality control (QC) samples were resuspended in 140 L of a 50% water/methanol solution, lysed, and centrifuged, and the supernatant was transferred to a new tube
Disturbing this redox balance influenced major virulence traits, including melanin and titan cell formation, dissemination to the brain and the extracellular composition of cells
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