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4 When considering only NF1-associated, radiation-exposed, and sporadic malignant peripheral nerve sheath tumors, previous studies showed complete loss of H3K27 trimethylation in variable proportions: 29% (12/42), 5 34% (55/162), 10 56% (30/54), 8 57% (51/90), 6 68% (17/25), 9 73% (45/62), 4 and 75% (47/63)

The medium was changed to a serum-free medium (D6046, Sigma-Aldrich) 48 h after seeding, and sodium selenite (100 nM, S9133, Sigma-Aldrich) was added at the same time for maintaining the expression of GPX3