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Its encoding gene is located on chromosome 11 and is composed of seven exons
It was first identified in human plasma and has since become a major subject of interest in regenerative biology, tissue engineering, and extracellular matrix research
Cells were plated in collagen-coated 96-well plates at the density of 2 10 4 per well in 0.1 mL DMEM
Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency