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Lay out every item in order of use before the vial is punctured
ER tracking assay HESC and 12Z cell lines were incubated with the compounds and labeled with and ER-Tracker Green (#E34251, Invitrogen, USA) (1 M) ( n = 3) in the dark for 30 min and analyzed by flow cytometry using a Becton Dickinson (San Jose, CA, USA) flow cytometer and a Beckman Coulter Cytoflex Cytometer Software (v 1.2.5.3891)
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still, their effectiveness is limited, and no therapeutic strategy is able to fully stop OA progression
* NMN - Nicotinamide Mononucleotide
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