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Cation-adjusted MHB medium (Oxoid) for susceptibility testing was prepared as per manufacturer recommendations

The trade-off is convenience

Symptomatic awareness of hypoglycemia may be less pronounced in patients with longstanding diabetes, in patients with diabetic neuropathy, using drugs that block the sympathetic nervous system (e.g., beta-blockers) [see Drug Interactions (7)], or who experience recurrent hypoglycemia

PMCID: PMC11944337

Permeabilization was then performed using PBS containing 0.5% Triton X-100 (108,603, Merck, Darmstadt, Germany) for 1 h at room temperature, followed by washing twice for 5 min in PBS/BSA