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The reaction mixtures were manually mixed using a 100 to 300 l multichannel pipette (set to 250 l) and the microplate was inserted into a BioTek H1 Synergy microplate reader equilibrated to 37 C and vigorously shaken for 1 min followed by a 3.5-min delay

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Endothelial cell-surface gp60 activates vesicle formation and trafficking via G(i)-coupled Src kinase signaling pathway

This may play a role in glaucoma pathogenesis

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