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Prior to transfer, stain-free gels were imaged on a Biorad Chemidoc MP Imaging System (Biorad, Hercules, CA) to confirm equal protein load, and were then wet transferred onto PVDF membranes at 100 V constant for 1 h and equal transfer was routinely confirmed by Ponceau S stain

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Bacterial identification and susceptibility testing were performed by each contributing microbiology laboratory using Vitek MS and/or Vitek 2 ID & AST cards (N808/XN15) (bioMrieux, Durham, NC, USA) with the exception of one laboratory (Chantilly, VA)

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Reactive oxygen species-induced autophagic degradation of Helicobacter pylori CagA is specifically suppressed in cancer stem-like cells