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The general thiol reagent, 5-5'-dithiobis[2-nitrobenzoic acid] (DTNB, Ellman's Reagent) reacts with GSH to form the 412 nm chromophore, 5-thionitrobenzoic acid (TNB) and GS-TNB
while the age and IGF-BP-3 were inconsistent between Group 2 and Group 1&3 as Group 2 was healthy controls with normal height
Lyophilization is a freeze-drying process that removes water from the peptide, turning it into a stable powder
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