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Similar approaches have successfully generated cardiac spheroids using rat neonatal ventricular cardiomyocytes, human dermal fibroblasts, and human coronary microartery ECs in ultra-low attachment plates [253], as well as spheroids composed of human iPSC-derived cardiomyocytes, human cardiac fibroblasts, and HUVECs in an ultra-low attachment hanging drop system [254]
Furthermore, GCH1 is a rate-limiting enzyme that is synthesized from BH 4
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