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Upon 15 min incubation on ice, cell lysates were cleared by centrifugation for 15 min at 10,000 g and subjected to SDS-PAGE and Western blot analysis

The paper was in German, but it seems the researchers ramped up the stationary bike by 50 watts every two minutes until the subjects reached exhaustion

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Specifically engineered for higher biological potency in vitro, IGF-1 LR3 has an increased half-life and a binding aversion to native proteins within the body that make it ideal for both research and large-scale culturing