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This makes it of particular interest in studies targeting body recomposition and long-term metabolic efficiency, where muscle retention is critical for sustaining basal metabolic rate

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Plasmid construction To generate CRISPR-Cas9 plasmids for gene manipulation in AG-haESCs, specific sgRNAs were synthesized, annealed, and inserted into the BpiI (Thermo Fisher Scientific, Waltham, MA, USA)-linearized pX330-mCherry plasmid (Cat# 98750

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