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Separate-Vial Reconstitution Spec BAC water added BPC-157 (10 mg vial) 2.0 mL TB-500 (10 mg vial) 2.0 mL Spec Final concentration BPC-157 (10 mg vial) 5.0 mg/mL TB-500 (10 mg vial) 5.0 mg/mL Spec 10 units (0.10 mL) delivers BPC-157 (10 mg vial) 500 mcg TB-500 (10 mg vial) 500 mcg Spec 20 units (0.20 mL) delivers BPC-157 (10 mg vial) 1.0 mg TB-500 (10 mg vial) 1.0 mg Spec Doses per vial (typical) BPC-157 (10 mg vial) ~2040 days TB-500 (10 mg vial) ~45 loading doses This separate-vial row keeps the same 10 mg-per-compound assumption as the 20 mg pre-blend, but the compounds are reconstituted and drawn independently

If any step breaks down (low stomach acid, lack of intrinsic factor, or gut inflammation), much of the B12 passes through unabsorbed

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Adjust the draw volume accordingly

In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min