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Parasite cultures were centrifuged, the pellets resuspended with 20 volumes of saponin lysis buffer (0.02% saponin, 10 mM NaH 2 PO 4 , 10 mM Na 2 HPO 4 , 145 mM NaCl, 3 mM KCL, pH 7.2), incubated for 10 min at 37C, and washed three times with phosphate-buffered saline

Briefly, islets were prepared by injecting collagenase (10 mL of liberase at 0.23 mg/mL) into the pancreatic duct followed by surgical removal of the pancreas

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Continue monitoring During GLP-1 receptor agonist therapy, monitor patients for the following signs or symptoms of acute pancreatitis: Weight loss of more than 1.5 kg per week is usually associated with prolonged periods of fasting or anorexia and, as discussed earlier, causes the release of cholesterol into the bile and induces bile stasis